Trichilemmoma.Examination by nucleus DNA quantity (Cytofluorometry).

HLA-B27 typing by use of flow cytofluorometry.

We describe the use of flow cytofluorometry to type lymphocytes for HLA-B27 antigens. We modified the cytotoxicity test of Terasaki and McClelland [Nature (London) 1964;204:998] for use with a flow cytofluorometer. In a second assay we used a commercially available monoclonal anti-HLA-B27 antibody and indirect immunofluorescence staining of cell surfaces. Although this antibody cross reacts wit...

Re-evaluation of the quantity of DNA in cell nucleus of two animal species by microspectrophotometry and autoradiography.

[Nuclear DNA cytofluorometry of normal human laryngeal epithelia and squamous cell carcinoma].

Normal human laryngeal epithelia and laryngeal squamous cell carcinoma were assayed by Feulgen DNA cytofluorometry using free cell nuclei isolated from carnoy-fixed, paraffin-embedded specimens. In all of the 12 normal specimens, the epithelium showed typical diploid cell clones with low proliferative activity. Polyploid cells were seen in only two specimens from subjects aged 61 and 69 years r...

Quantity Discrimination by Zebrafish

Discrimination of quantity (magnitude) was investigated in zebrafish (Danio rerio). Male zebrafish chose to approach the location previously occupied by the larger in number between 2 groups of female conspecifics (no longer visible at test) in sets of 1 versus 2 items, and 2 versus 3 items, but failed at 3 versus 4 items; similarly, when tested with larger numbers, zebrafish succeeded with 2 v...

comparison of quantity and quality of different dna extraction methods for lysiphlebus species by spectrophotometer

dna extraction with an appropriate quality and quantity is the first step in molecular identification of some group of insects that their identification morphologically is difficult and dependent on a particular life stage, size or gender. selection of a suitable protocol is a key factor to provide a pure dna. in this study quality and quantity of five extraction methods used in entomological l...